FIG. 7.

The BMV proteins do not have potent silencing suppressing activity. (A) Microscopic images of tobacco leaves infiltrated with GFP plus dsGFP, which will induce posttranscriptional silencing, silencing suppressors, and BMV proteins. The upper panel of three images shows the controls where there is silencing and the reversal of silencing by P19 and 2b. The images were taken with a Zeiss Axioplan 2 microscope using an FITC filter (at a 543-nm excitation and a 505- to 530-nm emission). The images were processed with Adobe systems software. (B) Quantitative analysis of GFP expression in plant lysates. The emissions are measured in arbitrary fluorescent units but can be compared to each other.