FIG. 9.

BMV proteins and the stabilities of BMV RNAs. (A) Effects of BMV replication proteins on transfected BMV RNA3. The gel images are all from one experiment examining the stability of radiolabeled BMV RNA3 transfected into tobacco protoplasts that express GFP, 1a, or 2a. Total RNAs were harvested at 0 to 6 h posttransfection and then electrophoresed in an agarose gel. The half-lives of the RNAs were determined by deriving the time in which half of the initial amount of RNA was present. (B) The effects of BMV RNAs transfected into barley protoplasts. The transcripts transfected into protoplasts are identified above and to the right of the gel image. The times (in hours) when the RNAs were harvested after inoculation of the protoplasts are noted below the gel image. (C) Accumulations of the BMV RNAs in barley protoplasts transfected with all three BMV RNAs. This gel image is identical to the last two lanes of the gel in panel C. However, it was exposed for a shorter period of time to allow visualization of the replication products. hpi, hours postinfiltration.