FIG. 5.
Products of in vitro translation and proteolytic processing of poliovirus RNAs in HeLa and SK-N-MC cell extracts. Transcript RNAs derived from PV1(M), sPV1(M), and their derivatives were translated and analyzed in HeLa or SK-N-MC cell extracts as indicated (see Materials and Methods). Differences in translation efficiencies among mutant RNAs in HeLa or SK-N-MC cell extracts were revealed by the percentage of VP3 protein generated by translation in each cell extract relative to that obtained for PV1(M) (100%). Bands correspond to segments of the poliovirus polyprotein following proteolytic processing. VPO, 2BC, 3AB, and 3CDpro are precursor polypeptides.