TABLE 2.
The sequence used for MRMP detectiona
| Name | Sequence (5′−3′) |
|---|---|
| gDNA0(23S rRNA) | TGAAGACACCCGTTAG |
| gDNA1(23S rRNA-WT) | AACGAAACGGAAAGAC |
| gDNA1(23S rRNA-MT) | AACGAAACGGGAAGAC |
| gDNA2(23S rRNA-WT) | AACGGGACTTAAAGAC |
| gDNA2(23S rRNA-MT) | AACGGGACTTGAAGAC |
| gDNA3(23S rRNA-WT) | AACGGGACAAAAAGAC |
| gDNA3(23S rRNA-MT) | AACGGGACAAGAAGAC |
| 23S rRNA-forward | ATCCAGGTACGGGTGAAGAC |
| 23S rRNA-reverse | GTCCTGATCAATATTAAGCTACAGT |
| 23S rRNA -probe | CCCGTTAGGCGCAACGGGACGG |
| ssDNA (23S rRNA-WT) | GCTTCACGGGGTCTTTCCGTCCCGTTGCGCCTAACGGGTGTCTTCACCCGTACCTGGAT |
| ssDNA (23S rRNA-MT) | GCTTCACGGGGTCTTCCCGTCCCGTTGCGCCTAACGGGTGTCTTCACCCGTACCTGGAT |
a
Bold was used to highlight the nucleotide at position 2063 for clear identification of WT versus MT sequences, and bold/italics were employed to indicate the positions and types of introduced mismatches.