FIG. 4.
Localization of EGFP-tagged RPVKO polymerase and the H glycoprotein using CSLM. B95a cells were infected at an MOI of 0.01 with rRPVKOL-RRegfpR for 24 h. RPV H was detected on the surface of nonpermeabilized cells using a mouse monoclonal antibody and visualized by indirect immunofluorescence using an Alexa Fluor 568-conjugated secondary antibody (red). EGFP was visualized by virtue of its autofluorescence (green). Nuclei were counterstained with DAPI (blue). (A) Photomicrograph of a syncytium comprised of eight infected cells. Magnification, ×1,432. (B) Composite photomicrograph of singly infected cells. Magnification, ×1,841. The inset is a magnification of a small region of the cell membrane indicated by the box.