FIG. 6.

(A) Kinetics of alkylation-induced fusion inhibition. Virus, prebound to XC cells, was subjected to alkylation for 0 to 40 min by incubation at 37°C in fusion buffer containing 1.2 mM M135 and then further incubated in the absence of alkylator for a total time of 40 min. After the cells were washed with virus inactivation buffer, fused cells were allowed to rearrange into polykaryons. Fusion efficiencies relative to that of a control sample incubated for 40 min without alkylator were calculated as described in the legend to Fig. 1C. (B) Correlation between the kinetics of alkylation-induced fusion inhibition and fusion of Mo-MLV. Note that the kinetics of alkylation-induced fusion inhibition is represented as its inverse curve. The fusion kinetics is from Fig. 1C.