FIG. 5.

hBD1 and hBD2 do not modulate expression of CXCR4 on the cell membrane. Uninfected PBMC were treated with hBD2, and expression of CXCR4 was evaluated by flow cytometry. PBMC were treated with hBD1 and hBD2 for 2, 5 and 20 h and then labeled with FITC-CD4, PerCP-CD3, and PE-CXCR4; cells were then washed and fixed in 1% paraformaldehyde solution for flow cytometric analysis using a FACSCalibur flow cytometer. Live cells were gated according to forward and side scatter profiles. Data analysis was performed using FlowJo software. Shown here are representative results, after 2 h of treatment, of three independent experiments.