FIG. 4.

Inhibition of p38 MAPK does not affect CVB3 viral protein synthesis. (A) HeLa cells were preincubated with p38 inhibitor SB203580 for 30 min and then infected with CVB3 (MOI = 10) for 6 h. Cell lysates were collected and immunoblotted with antibodies against phospho-hsp27 (p-hsp27) and phospho-ATF-2. hsp27 was probed as the loading control for p-hsp27, and β-actin was probed as the loading control for p-ATF-2. Phosphorylation of hsp27 and ATF-2 was quantitated by densitometric analysis using ImageJ (National Institutes of Health, version 1.32j) and normalized to control levels (CVB3-infected cells without inhibitor) arbitrarily set to 100%. Results are means ± SD (n = 3). *, P < 0.05 compared to nontreated cells. (B) HeLa cells were infected in the absence or presence of the p38 inhibitor SB203580 as described above. Cell lysates were collected to examine the expression of viral capsid protein VP1 and the phosphorylation of JNK1/2. β-actin was probed as the loading control. Results are means ± SD (n = 3). p-, phospho-.