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. 2005 Nov;25(22):9920–9935. doi: 10.1128/MCB.25.22.9920-9935.2005

FIG. 7.

FIG. 7.

FIG. 7.

CRMP-2-dependent localization of Sra-1 and WAVE1 at the growth cones of axons. (a and b) Effect of expression of CRMP-2 D71N on the localization of Sra-1 and WAVE1 at the growth cones of axons. pCAGGS-myc-GST, CRMP-2 WT, CRMP-2 D71N, or CRMP-2 ΔN440 was transfected into hippocampal neurons. Transfected cells were stained with anti-myc monoclonal and anti-Sra-1 or anti-WAVE1 polyclonal antibodies. The percentage of accumulation of Sra-1 and WAVE1 at the growth cones of axons was estimated. (c) Effect of the knockdown of CRMP-2 on the localization of Sra-1 and WAVE1 at the growth cones of axons. The indicated Cy3-labeled siRNA and pCAGGS-myc-GST were cotransfected into hippocampal neurons. More than 80% of the cells transfected with Myc-GST were Cy3-siRNA positive. Transfected cells were stained with anti-myc monoclonal and anti-Sra-1 or anti-WAVE1 polyclonal antibody at DIV3. The accumulation of Sra-1 and WAVE1 at the growth cones of axons was estimated. The data are means ± standard deviations of at least three independent experiments. Asterisks indicate the difference from the value of GST at P < 0.05 (Student's t test). n > 150. Bar, 20 μm.