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The Pds gene is induced by forskolin, serum, and TSH in thyroid cells. FRTL-5 cells were starved of calf serum (CS) and six hormones (6H) for 72 h (CS, 0.2%) and then stimulated with forskolin (FSK; 10 μM) for 12 h before harvesting or starved of six hormones (CS, 5%) and then stimulated with five hormones (5H) or five hormones and TSH for 12 h. Pds mRNA levels were monitored by real-time PCR as previously described. Data are the means of three separate experiments.
