FIG. 1.

The MFA2 3′-UTR can regulate translation through its AU-rich region. Luc-MFA2, Luc-DII, and Luc-TNF-α reporter translation compared to the Luc-PGK1 reporter in wild-type cultures. The Luc-MFA2 reporter bears the entire 3′-UTR, the Luc-TNF-α reporter bears the 34-nucleotide AU-rich region of TNF-α 3′-UTR (49), and the domain II reporter lacks the AU-rich region of MFA2 3′-UTR (35). All luciferase values were normalized to mRNA abundance and compared to the Luc-PGK1 luciferase activity in the wild-type strain, and the assay was repeated at least three times.