FIG. 3.

ARE-controlled translation is regulated by carbon source growth conditions. The 3′-UTRs of PGK1, MFA2, and TIF51A and the domain II construct which lacks the AU-rich domain of the MFA2 3′-UTR were tested for luciferase activity using firefly reporters as described in Materials and Methods. Shown is Luc-PGK1, Luc-MFA2, Luc-TNF-α, Luc-TIF51A, and Luc-DII reporter translation in wild-type glucose- and glycerol-grown cultures compared to the Luc-PGK1 reporter in wild-type glucose-grown cultures. All values are compared to the Luc-PGK1 reporter activity in the WT strain grown in glucose conditions and are normalized for RNA levels. The luciferase experiment was repeated three times.