FIG. 2.

Functionally normal gonadotropes of AhR^−/− pituitaries. (A) Presence of gonadotrophs in the pituitary anterior lobes of AhR^−/− females. Cryosections of pituitaries isolated from AhR^+/+ and AhR^−/− mice were treated with an anti-LH antibody, which should specifically stain pituitary gonadotrophs. A and P represent the anterior and posterior lobes, respectively. Bar, 0.1 mm. (B) Secretion of LH from the gonadotrophs of AhR^−/− pituitaries. A GnRH agonist, buserelin (2 μg), or vehicle alone was injected subcutaneously into ovariectomized AhR^+/+ and AhR^−/− females. One hour after injection, serum levels of LH were determined. Values are represented as means ± SD for three to four mice.