Expression profiles for RBM4 and α-TM mRNA isoforms across selected human tissues. (A) Northern blot analysis of human RBM4. (B) Total RNAs from different human tissues were subjected to RT-PCR analysis using primers specific to RBM4 (primer set 15) (Table 1), three α-TM isoforms (primer set 1 for sk, 2 for sm, and 3 for 9a′) and GAPDH (primer set 16). The lower panel depicts gene structure of human α-TM and its splice isoforms and primers used in RT-PCR. Closed and hatched boxes represent sk- and sm-specific exons, respectively. *, translation stop codon; pA, polyadenylation signal.