TABLE 1.

Characteristics of sets of PCR primers^a

Set no.	Gene product	Accession no.	Forward primer	Reverse primer	PCR product size
1	α-Tropomyosin	NM_000366	725-741b	1009-1024b	347 bp
2			725-741	51-69 of exon 9db	162 bp
3			725-741	17-34 of intron 9ab	206 bp
4			820-838b	1009-1024	254 bp
5			820-838	17-34 of intron 9a	113 bp
6			SV40c	1009-1024
7			SV40c	β-TM P4c
8			SV40c	241-264b
9	Calmodulin 1	NM_006888	77-94	393-409
10	Flotillin 1	NM_005803	20-35	360-376
11	Caldesmon 1	NM_033138	62-77	531-547
12	Rho C	NM_175744	25-41	432-448
13	RPL27a	NM_000990	10-27	429-446
14	RIPK2	NM_003821	34-51	761-777
15	RBM4	NM_002896	587-601	1018-1037
16	GAPDH	NM_002046	18-41	300-325

^aPCR primers were designed from the sequences of the genes having the NCBI accession number listed. The sequences of α-TM exon 9d and intron 9a were derived from NCBI accession number NT_010194.

^bPrimers used for detection of α-TM were derived from exon 8 (725-741 of NM_000366; AACAATTAAGAATAATG), exon 9a (820-838 of NM_000366; TCAGCTTGTCGGAAAGGAC), exon 9b (1009-1024 of NM_000366; GGTGTAAGCAGGCAGA), exon 9d (51-69 of exon 9d; CTGCGCCACATTCTCTCG), intron 9a (17-34 of intron 9a; CATGCCTTCCTTGCTCCC), and exon 3 (241-264 of NM_000366; see reference 12).

^cThe SV40 and β-TM P4 primers were previously described (20).