FIG. 1.

Infection of murine bone marrow macrophages with M. avium 101 activates p38 MAPK. (A) Western blot analysis of p38 phosphorylation (pp38) was performed following treatment of bone marrow macrophages with LPS or infection with complement-opsonized zymosan (COZ), complement-opsonized M. avium 101 (CO101) or nonopsonized M. avium 101 (NO101) or left untreated as resting cells (RC). Equal protein, as determined by the Micro BCA assay, was loaded in each lane. All blots were stripped and reprobed with a p38 antibody to show equal amounts of this protein, but only the 24-h total p38 blot is shown here. The relative densities of the 24-h phosphorylated p38 bands were analyzed by densitometry. AU, arbitrary units. (B) NOS2 expression from cell lysates was detected by Western blotting. Results are representative of three separate experiments.