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. 2002 Jun;70(6):3040–3052. doi: 10.1128/IAI.70.6.3040-3052.2002

FIG. 6.

FIG. 6.

p38 and ERK1/2 MAPKs are required for NOS2 and TNF-α production by pathogenic and nonpathogenic mycobacteria. (A) Western blot analysis of NOS2 production and phosphorylated ERK1/2 in macrophages pretreated with 25 μM SB203580 and/or 50 μM PD98059. The treated and control macrophages were infected for 4 h with M. avium 101 or M. smegmatis. Blots were probed for total ERK1/2 to show equal protein loading. (B) TNF-α in the culture supernatant was analyzed by ELISA. a, P < 0.01 compared to M. avium 101 plus DMSO; b, P < 0.001 compared to M. avium 101 plus DMSO; c, P < 0.05 compared to M. smegmatis. plus DMSO; d, P < 0.001 compared to M. smegmatis. Results are representative of three separate experiments.