FIG. 1.

Comparative levels of PcrH and LcrH produced in trans by the Y. pseudotuberculosis ΔlcrH null mutant. Immunoblot of chaperone protein prepared from bacteria grown in Yop-inducing medium (BHI minus Ca²⁺) supplemented with a range of IPTG concentrations (0 to 0.4 mM). Both PcrH and LcrH were identified using monoclonal anti-FLAG antiserum in combination with enhanced chemiluminescence detection. Also indicated is the concentration of IPTG required to generate enough LcrH for trans-complementation of the ΔlcrH null mutant with respect to regulation. Definitions of TS (temperature sensitive) and CD (calcium dependent) can be found in Table 3.