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. 2005 Nov;187(22):7738–7752. doi: 10.1128/JB.187.22.7738-7752.2005

FIG. 10.

FIG. 10.

Analysis of Yop synthesis and secretion from Y. pseudotuberculosis strains grown either with (+) or without (−) Ca2+. The protocol is essentially the same as that described in the legend to Fig. 8. Lanes: a and b as well as i and j, wild-type YPIII/pIB102; c and d, yscX yscY null mutant YPIII/pIB881; e and f, complemented YPIII/pIB881, pJEB340 (YscX, YscY+); g and h, complemented YPIII/pIB881, pJEB335 (Pcr3, Pcr4+); k and l, lcrH yscX yscY null mutant YPIII/pIB881-650; m and n, complemented YPIII/pIB881-650, pPJE020 (LcrH+), pJEB340 (YscX YscY+); o and p, complemented YPIII/pIB881-650, pKEC005 (PcrH+), pJEB335 (Pcr3 Pcr4+). Molecular masses shown in parentheses are deduced from primary sequences.