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. 2005 Nov;187(22):7738–7752. doi: 10.1128/JB.187.22.7738-7752.2005

FIG. 4.

FIG. 4.

Analysis of Yops and LcrV synthesis and secretion from Y. pseudotuberculosis strains grown either with (+) or without (−) Ca2+. Yops and LcrV in the total protein fraction (a mix of proteins secreted to the culture medium and contained within intact bacteria) (upper panel) or secreted to the extracellular medium (cleared culture supernatants) (lower panel) were separated by SDS-PAGE and identified by immunoblot analysis using polyclonal rabbit anti-YopH, anti-LcrV, and anti-YopD antiserum. Where indicated, IPTG was added at a final concentration of 0.4 mM upon temperature shift. Lanes: a and b, wild-type YPIII/pIB102; c and d, lcrH null mutant YPIII/pIB650; e and f, complemented YPIII/pIB650, pJEB133 (LcrH+); g and h, complemented YPIII/pIB650, pJEB121 (Hyb1+); i and j, complemented YPIII/pIB650, pJEB122 (Hyb2+); k and l, complemented YPIII/pIB650, pJEB123 (Hyb3+); m and n, complemented YPIII/pIB650, pJEB124 (Hyb4+); o and p, complemented YPIII/pIB650, pJEB125 (Hyb5+); q and r, complemented YPIII/pIB650, pJEB126 (Hyb6+); s and t, complemented YPIII/pIB650, pJEB127 (Hyb7+); u and v, complemented YPIII/pIB650, pJEB128 (Hyb8+); x and y, complemented YPIII/pIB650, pJEB129 (Hyb9+); z and aa, complemented YPIII/pIB650, pJEB199 (Hyb10+); bb and cc, complemented YPIII/pIB650, pJEB132 (PcrHNEISS+); dd and ee, complemented YPIII/pIB650, pJEB130 (PcrH+). Molecular masses shown in parentheses are deduced from primary sequences.