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. 2005 Nov;187(22):7738–7752. doi: 10.1128/JB.187.22.7738-7752.2005

FIG. 7.

FIG. 7.

Infection of HeLa cells by P. aeruginosa. Strains were allowed to infect a monolayer of growing HeLa cells. At 3 h after infection, the effect of the bacteria on the HeLa cells was recorded by phase-contrast microscopy. Note the extensive rounding up of the ExoS-dependent, cytotoxically affected HeLa cells (A, C, D, F, and G). HeLa cells infected with the Δpcr3 or Δpcr4 mutant show normal cell morphology (compare B and E with H), even after prolonged infection for up to 6 h (data not shown). Shown are phase-contrast images of cells infected with wild-type PAK (A), Δpcr3 mutant PAKpcr3 (B), trans-complemented PAKpcr3, pJEB295 (Pcr3+) (C), trans-complemented PAKpcr3, pJEB291 (YscX+) (D), Δpcr4 mutant PAKpcr4 (E), trans-complemented PAKpcr4, pJEB296 (Pcr4+) (F), trans-complemented PAKpcr4, pJEB292 (YscY+) (G), or cells left uninfected (H).