FIG. 1.

Protein sequence analysis of the 65-kDa surface protein. A. Coomassie blue-stained 10% SDS-polyacrylamide gel of S. mutans surface proteins. Lane 1, OMZ175; lane 3, A32-2; lanes 2 and 4, molecular mass markers (size in kilodaltons indicated on right). Asterisks indicate positions of the 40-kDa and 65-kDa homologs. The 65-kDa band from A32-2 was excised from the gel and sequenced. B. Two 11-aa sequences were obtained by CNBr sequencing of the 65-kDa surface protein from S. mutans A32-2 and were found to contain 78% identity to the homologous sequences from both OMZ175 and UA159. Changes from the UA159 sequence are in italics.