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Derepression of the cysH operon in a cysK background is not due to OAS accumulation. Strains 1A3-530 (cysE, PcysH-lacZ) and DAOK-530 (cysE, PcysH-lacZ, and cysK) were grown and recovered by centrifugation as described in Fig. 2. Washed cells were then inoculated in sulfur-free minimal media supplemented with cystine or cystine plus OAS. Samples were taken 4 h after resuspension. β-Galactosidase activities are expressed in MU.
