TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| Bacillus subtilis | ||
| JH642 | trpC2 pheA1 | Laboratory stock. |
| JH642K | JH642 cysK::mini-Tn10; Spr Cmr | This study |
| DA642X | JH642 amyE::pJM116X; Cmr | This study |
| DA642X-K | DA642X cysK::mini-Tn10; Spr Cmr | This study |
| MC530 | JH642 amyE::PcysH-lacZ | 21 |
| 1A3 | cysE trpC2 purA26 | BGSCb |
| 1A3-530 | 1A3 amyE::PcysH-lacZ | 21 |
| SB11 | Sp100 BS168 cysK::mini-Tn10; Spr | 37 |
| MCK530 | MC530 cysK::mini-Tn10; Spr Cmr | This study |
| DAOK-530 | 1A3-530 cysK::mini-Tn10; Spr Cmr | This study |
| MCK530-DK1 | MCK530 thrC::Pxyl-cysK; Spr Cmr Emr Lmr | This study |
| MCK530-C795 | MCK530 thrC::pDG795; Spr Cmr Emr Lmr | This study |
| Escherichia coli DH5α | supE44 thi-1 ΔlacU169(φ80lacZΔM15) endA1 recA1 hsdR17 gyrA96 relA1 trp6 cysT329::lac inmλp1(209) | Laboratory stock |
| Plasmids | ||
| pJM116 | Integrative vector to construct transcriptional fusions to lacZ; integrates at the amyE locus of B. subtilis; Cmr | 6 |
| pJM116X | Contains the xylose promoter of plasmid pRDC9 cloned in the EcoRI-HindIII sites of pJM116 | 21 |
| pDG795 | Integrates at the thrC locus of B. subtilis; Emr Lmr | 13 |
| pGES45 | Xylose promoter and repressor cloned into pDG795 | G. Schujman, personal communication |
| pDK1 | cysK cloned under Pxyl in pGES45 | This study |
a
Cmr, Emr, Lmr, and Spr denote resistance to chloramphenicol, erythromycin, lincomycin, and spectinomycin, respectively.
b
Strain obtained from the Bacillus Genetic Stock Center.