TABLE 2.
Expression profile of sulfur metabolism-related genes in strains JH642 and JH642K
| Gene | Localization (°) | Function and/or similarity | Ratio
|
|
|---|---|---|---|---|
| JH642a Glut/Cys | JH642K/JH642b | |||
| cysS | 9 | Cysteinyl-tRNA synthetase | (1.3)c | (1) |
| cysE | 9 | Serine acetyltransferase | (1.3) | (1) |
| yvfD | 300 | Similar to serine acetyltransferase | (−) | (0.9) |
| cysH | 139 | PAPS reductase | 4.7 | 7 |
| cysP | 139 | Sulfate permease | 5 | 4 |
| sat | 139 | Similar to ATP sulfurilase | 9 | 13 |
| cysC | 139 | Similar to APS kinase | 9 | 15 |
| ylnD | 139 | UROIII methyltransferase | 9 | 15 |
| ylnF | 139 | Ferrokelatase | 9 | 5 |
| cysJI | 293 | Similar to sulfite reductase | 3 | 6 |
| cysL | 330 | LysR transcriptional regulator | (0.7) | (0.5) |
| cysK | 7 | OAS-thiol liase | 5.5 | 3 |
| yrhA | 238 | Similar to OAS-thiol liase | 14 | 14 |
| ytkP | 262 | Similar to OAS-thiol liase | (−) | (1) |
| yisZ | 100 | Similar to APS kinase | 2.7 | (0.7) |
| yitA | 100 | Similar to ATP sulfurilase | (1.7) | (0.6) |
| yitB | 100 | Similar to PAPS reductase | (1.7) | (0.6) |
| ykuP | 127 | Similar to sulfite reductase | (0.6) | (1.2) |
| yvdB | 304 | Similar to SulP permease | (−) | (0.8) |
| ybaR | 15 | Similar to SulP permease | (−) | (0.9) |
| ssuBACD | 82 | Sulfonates utilization | 7.7 | 12 |
| ytlI | 257 | LysR transcriptional regulator | 4 | 4 |
| ytmI | 257 | Unknown | 13 | 13 |
| tcyJ | 257 | l-Cystine and sulfur compounds ABC transporter | 12 | 10 |
| tcyK | 257 | l-Cystine and sulfur compounds ABC transporter | 15 | 10 |
| tcyL | 257 | L-Cystine and sulfur compounds; ABC transporter | 7.8 | 16 |
| tcyM | 257 | L-Cystine and sulfur compounds; ABC transporter | 5 | 8 |
| hisP | 257 | Histidine transporter | 16 | 15 |
| ytmO | 257 | Similar to monooxygenase | 16 | 22 |
| ytnI | 257 | Unknown | 16 | 14 |
| ytnJ | 256 | Similar to nitrilotriacetate monooxigenase | 16 | 20 |
| ribR | 256 | Riboflavin kinase | 16 | 11.5 |
| hipO | 256 | Hippurate hydrolase | 10 | 6 |
| ytnM | 256 | Unknown | 10 | 7 |
| tcyP | 84 | l-Cystine symporter | 2.5 | 6 |
| tcyA | 35 | l-Cystine ABC transporter | (1.0) | 3 |
| yrzC | 240 | Negative regulator of ytmI operon | (0.8) | (0.9) |
a
Ratio of expression of genes in wild-type cells grown in the presence glutathione (Glut) as a sulfur source compared to wild-type cells grown in the presence of cystine (Cys) as a sulfur source. The average ratios of three independent experiments are shown.
b
Ratio of expression of genes in cysK mutant cells grown in the presence of cystine as sulfur source supplemented with casein hydrolysate and yeast extract compared to wild-type cells grown in the same medium. The average ratios of three independent experiments are shown.
c
Values in parentheses indicate genes not significantly induced or repressed.