TABLE 2.

Activities of the AT proteins of C. glutamicum with detectable activities toward branched-chain amino acid intermediates or 2-oxo-butyrate

ATa	Amino donorb	Amino acceptor	Sp actc (μmol min−1 mg of protein−1)
ArgD	l-Glu	O-But	0.1
	l-Ala	O-But	0.1
	l-Asp	O-But	0.1
	l-Gln	O-But	0.1
IlvE	l-Glu	O-Ile	9.6
	l-Glu	O-Leu	13.9
	l-Glu	O-Val	13.7
	l-Glu	O-But	4.3
AvtA	l-Ala	O-Ile	3.7
	l-Ala	O-Leu	0.9
	l-Ala	O-Val	18.2
	l-Ala	O-But	27.5
	l-Gln	O-Ile	0.1
	l-Gln	O-Leu	0.1
	l-Gln	O-Val	0.1
	l-Gln	O-But	0.1
AlaT	l-Glu	O-But	5.4
	l-Ala	O-But	3.0
	l-Asp	O-But	2.3
	l-Gln	O-But	0.7
NCgl0780	l-Glu	O-Leu	0.1
	l-Glu	O-But	0.2
AroT	l-Glu	O-Leu	1.3
	l-Ala	O-Leu	0.8
	l-Asp	O-Leu	0.1
	l-Gln	O-Leu	0.1
	l-Glu	O-Val	0.1
	l-Ala	O-Val	0.1
	l-Asp	O-Val	0.1
	l-Gln	O-Val	0.1
	l-Glu	O-But	1.1
	l-Ala	O-But	0.7
	l-Asp	O-But	0.1
	l-Gln	O-But	0.1
HisC	l-Glu	O-Leu	0.8
	l-Ala	O-Leu	0.1
	l-Asp	O-Leu	0.4
	l-Gln	O-Leu	0.1
	l-Glu	O-But	0.2
	l-Ala	O-But	0.1
	l-Asp	O-But	0.1
	l-Gln	O-But	0.1
NCgl0462	l-Glu	O-But	0.1
	l-Ala	O-But	0.1
	l-Gln	O-But	0.1

^aEither names or NCgl numbers are given. See also Table 4.

^bAmino donors are given as their three-letter code; amino acceptors are as follows: O-Ile, 2-oxo-3-methylvalerate; O-Leu, 2-oxo-isocaproate; O-Val, 2-oxo-isovalerate; and O-But, 2-oxo butyrate.

^c0.1 means detectable amino acid formation compared to the other 12 ATs not included in the table. Each value represents the average of at least two independent assays.