TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant features | Reference |
|---|---|---|
| Strains | ||
| E. faecalis | ||
| FA2-2 | rif fus | 12 |
| JH2SS | spc str | 37 |
| UV202 | fir fus, recombination-deficient mutant of JH2-2 | 46 |
| OG1X | str | 20 |
| OG1RF | rif fus | 8 |
| OG1SS | spc str | 12 |
| E. faecium | ||
| BM4105RF | rif fus | 21 |
| BM4105SS | spc str | 21 |
| E. coli DH5α | endA1 recA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δ(argE-lacZYA)U169 | Bethesda Research Laboratories |
| Plasmids | ||
| pHTα | pHTβ carrying IS232-like element | 42 |
| pHTβ | pMG1-like vancomycin resistance (Tn1546) conjugative plasmid | 42 |
| pHTγ | pHTβ carring group II intron | 42 |
| pAM401 | E. coli-E. faecalis shuttle; cat tet | 45 |
| pTV32Ts | Transposon delivery vector, temperature sensitive; pE194Ts(Cmr)::Tn917-lac(Emr) | 31 |
| pMW119 | E. coli cloning vector; Ampr | Nippon Gene |
| pBluescript SKII(+) | E. coli cloning vector; Ampr | Stratagene |
| pACYC184 | E. coli cloning vector; Tcr Cmr | New England Biolabs |
| pHTβ/ORF34del | pHTβ derivative mutant carrying a deletion of motif III of ORF34 (Tral) | This study |