TABLE 2.

Open reading frames identified in pHT plasmids^a

ORF	5′ end	3′ end	Amino acid size	Identification [organism]	Protein family/domain	% Identity
1	52467	1946	787	Trsl (Tral) [L. lactis]/pXO2-77/76 [B. anthracis]	DNA topoisomerase I/III	34/29
2	2264	2863	199	AbiQ [L. lactis]	Phage abortive infection mechanism	19
3	2984	3199	71	Hypothetical protein
4	3515	3721	68	Hypothetical protein
5	3801	4655	284	ParA [E. faecalis; pTEF3]/pXO2-39 [B. anthracis]	Replication-related protein	29/27
6	4668	5105	145	YvfU [B. sutilis]	Two-component sensor histidine kinase	19
7	5123	5422	99	Hypothetical protein
8	5425	5868	147	Hypothetical protein
9	6014	6337	107	Hypothetical protein
10	6755	10384	1,209	pXO2-28 [B. anthracis]	Putative cell surfrace protein	10
11	10444	10806	120	Hypothetical protein
12	10820	10972	50	Hypothetical protein
13	11079	11462	127	pXO2-26 [B. anthracis]		26
14	11474	12889	471	pXO2-25 [B. anthracis]	VirB11 family, secretory protein	40
15	12927	13721	264	pXO2-24/23 [B. anthracis]		29/12
16	13721	14518	265	pXO2-22 [B. anthracis]		43
17	14536	14898	120	pXO2-21 [B. anthracis]		51
18	14912	15058	48	Hypothetical protein
19	15310	15585	91	pXO2-18 [B. anthracis]		47
20	15694	17520	608	pXO2-17 [B. anthracis]	Chromosome segregation ATPases	10
21	17533	18249	238	Hypothetical protein
22	18262	21120	952	VirD4 [E. faecium]/pXO2-16/15 [B. anthracis]	VirD4/TraG/TraD family (coupling protein)	33/33/10
23	21138	23912	924	AidA [E. faecium]/pXO2-14 [B. anthracis]	Amino acid transporters	71/25
24	23960	24271	103	pXO2-11 [B. anthracis]		35
25	24268	24885	205	pXO2-10 [B. anthracis]		35
26	24902	25369	155	Hypothetical protein
27	25385	27340	651	TrsE (TraE) [L. lactis; pMRC01] pXO2-09 [B. anthracis]	VirB4 family (transfer complex protein)	22/48
28	27362	28498	378	ORF14 [E. faecalis; Tn916]/pXO2-08 [B. anthracis]	NLP/P60 family, cell wall-associated hydrolase	32/43
29	28512	29162	216	Hypothetical protein
30	29176	30072	298	pXO2-05 [B. anthracis]		25
31	30108	30338	76	Hypothetical protein
32	30689	30985	98	pXO2-04 [B. anthracis]	ABC transport system, permease component	22
33	30982	31449	155	Hypothetical protein
34	31532	33052	506	pXO2-84 [B. anthracis]	Three motifs conserved in DNA relaxase/nickase	21
35	33074	33403	109	Hypothetical protein
36	33405	33668	87	Hypothetical protein
37	33890	34756	288	HlyD [E.. coli]	Hemolysin secretion protein	11
38	34911	36737	608	LtrC [L. lactis; pMRC01]/pXO2-81 [B. anthracis]	Catalytic active site (HEXXH)	15/23
39	36799	37815	338	Hypothetical protein	ABC transpoter
40	37824	38081	85	Hypothetical protein
41	38122	38412	96	Hemolysin-related protein [Thermotoga maritima]		21
42	38437	39441	334	LtrC-like protein [S. epidermidis]/pXO2-78 [B. anthracis]		26/34
43	39532	39822	96	Hypothetical protein
44	39984	40175	63	Hypothetical protein
45	40284	40502	72	Hypothetical protein
46	40539	40856	105	Hypothetical protein
47	40867	41064	65	Hypothetical protein
48	41061	42029	322	Hypothetical protein	Predicted ATPase
49	42255	43442	395	Hypothetical protein
50	43553	43846	97	Hypothetical protein
51	44498	44905	135	Prophage ps3 protein 15 [L. lactis]	Cro/CI family, transcriptional regulator	21
52	45057	45668	203	PinR [E.. coli; lambdoid prophage Rac]	Site-specific recombinases, DNA invertase	33
53	46046	46219	57	Hypothetical protein
54	46387	46695	102	Hypothetical protein
55	46707	47024	105	Hypothetical protein
56	47191	47754	187	71ORF1 [E. faecium; pMG1]		100
57	47765	48625	286	traA [E. faecium; pMG1]	Related to formation of mating aggregate	98
58	49122	49376	84	Hypothetical protein
59	49773	51374	533	RepS [E. faecalis; V583]/pXO2-38 [B. anthracis]	Replication protein	34/27
60	51608	51907	99	PrgN [E. faecalis; pCF10]	Replication and negative control of conjugation	38
61	52041	52418	125	Hypothetical protein

^aThe colums list open reading frame number, 5′ end of the ORF, 3′ end of the ORF, deduced amino acid size, identification of similar proteins and organisms, functional protein family or conserved domain, and percentage identity of the pHT plasmid ORF to the matching protein(s).