TABLE 3.

Transfer of pHTβ::Tn917-lac derivatives

No. in Fig. 1 and 2	Representative plasmid	Location of Tn917-lac insertion	Position of insertion (bp)	Frequency of transfer (broth mating)a	Relative transfer frequency (broth mating)
Wild type	pHTβ	Wild type	Wild type	1.1 × 10−4	100
1	pHTβ::Tn917-lac/315	ORF1 (C terminal)	492	1.9 × 10−4	172
2	pHTβ::Tn917-lac/261	ORF2	2445	2.8 × 10−4	254
3	pHTβ::Tn917-lac/268	ORF8	5589	<1.1 × 10−7	<0.1
4	pHTβ::Tn917-lac/136	ORF10	8937	4.2 × 10−7	0.4
5	pHTβ::Tn917-lac/55	ORF10/ORF11	10425	<2.0 × 10−7	<0.2
6	pHTβ::Tn917-lac/154	ORF13	11171	<2.1 × 10−7	<0.2
7	pHTβ::Tn917-lac/60	ORF14	11798	<1.8 × 10−7	<0.2
8	pHTβ::Tn917-lac/148	ORF15	13221	<1.5 × 10−7	<0.2
9	pHTβ::Tn917-lac/328	ORF16	14123	<1.5 × 10−7	<0.2
10	pHTβ::Tn917-lac/331	ORF18/ORF19	15259	<1.7 × 10−7	<0.2
11	pHTβ::Tn917-lac/57	ORF20	16119	<1.5 × 10−7	<0.2
12	pHTβ::Tn917-lac/274	ORF23	23643	7.3 × 10−7	0.7
13	pHTβ::Tn917-lac/230	ORF27	25416	<2.0 × 10−7	<0.2
14	pHTβ::Tn917-lac/321	ORF29	29049	<2.2 × 10−7	<0.2
15	pHTβ::Tn917-lac/142	ORF30	29612	<1.7 × 10−7	<0.2
16	pHTβ::Tn917-lac/323	ORF31/ORF32 (oriT)	30573	<1.5 × 10−7	<0.2
17	pHTβ::Tn917-lac/275	ORF32	30821	<1.3 × 10−7	<0.2
18	pHTβ::Tn917-lac/278	ORF33	31343	<1.9 × 10−7	<0.2
19	pHTβ::Tn917-lac/95	ORF34	32626	<1.5 × 10−7	<0.2
20	pHTβ::Tn917-lac/250	ORF48/49	42073	8.5 × 10−5	77
21	pHTβ::Tn917-lac/203	ORF50/51	43864	9.1 × 10−5	83
22	pHTβ::Tn917-lac/22	ORF51	44414	1.4 × 10−4	127
23	pHTβ::Tn917-lac/309	ORF52	45297	9.5 × 10−5	86
24	pHTβ::Tn917-lac/304	ORF54	46466	4.8 × 10−5	44
25	pHTβ::Tn917-lac/82	ORF56	47472	<1.4 × 10−7	<0.2
26	pHTβ::Tn917-lac/10	ORF58	49350	8.0 × 10−5	73
27	pHTβ::Tn917-lac/19	ORF60	51712	1.3 × 10−4	118
28	pHTβ::Tn917-lac/210	ORF61	52052	1.7 × 10−5	15
29	pHTβ::Tn917-lac/17	ORF1 (N terminal)	52618	1.0 × 10−4	91

^aThe mating time was 4 h. The mating experiments were performed between E. faecalis OG1RF and OG1SS when the transconjugants were obtained by solid surface mating.