TABLE 4.

Mobilization frequencies of pAM401 derivatives (Cm^r) carrying the various segments of pHTβ by coresident pHTβ plasmid (Vm^r)

Segment in Fig. 1	Plasmids	5′/3′ end of segment on map (bp)	Length (bp)	Region	Transfer frequency (no. of conjugants/donor; broth mating)
					Vmr	Cmr
a	pHTβ/pAM401::ORF1	525/1300	776	ORF1 internal region	6.8 × 10−5	<10−7
b	pHTβ/pAM401::IR4	1657/3060	1,404	IR4 and ORF2	7.7 × 10−6	<10−7
c	pHTβ/pAM401::IR3	5618/6116	499	IR3	5.1 × 10−5	<10−7
d	pHTβ/pAM401::119/113	1300/12476	11,177	IR4 to ORF13	8.0 × 10−5	<10−7
e	pHTβ/pAM401::ORF19/21	15213/18648	3,436	ORF19, −20, and −21	4.6 × 10−5	<10−7
f	pHTβ/pAM401::119/96	20168/25254	5,087	ORF23, −24, and −25	5.3 × 10−5	<10−7
g	pHTβ/pAM401::IR5-1	29918/31161	1,244	IR5, ORF31 and −32	6.0 × 10−5	4.1 × 10−5
h	pHTβ/pAM401::ORF39/40	36696/38127	1,432	ORF39 and ORF40	5.7 × 10−5	<10−7
i	pHTβ/pAM401::IR1	39726/41121	1,396	IR1, ORF44 to −47	6.0 × 10−5	<10−7
j	pHTβ/pAM401::DR1	43218/44620	1,403	DR1 and ORF50	6.5 × 10−5	<10−7
k	pHTβ/pAM401::ORF51/52	44171/45708	1,538	ORF51 and ORF52	4.3 × 10−5	<10−7
l	pHTβ/pAM401::ORF53/56	45810/47759	1,950	ORF53 to ORF56	8.2 × 10−5	<10−7
m	pHTβ/pAM401::traA	47762/48505	744	traA internal region	5.2 × 10−5	<10−7
n	pHTβ/pAM401::IR2	48482/50108	1,627	IR2 and ORF58	9.1 × 10−6	3.4 × 10−6
o	pHTβ/pAM401::ORF58	48943/50108	1,166	ORF58	6.5 × 10−5	<10−7
p	pHTβ/pAM401::rep5′	49257/50108	852	Upstream region of rep	5.0 × 10−5	<10−7
q	pHTβ/pAM401::ORF60/61	51124/52626	1,503	ORF60 and ORF61	7.5 × 10−5	<10−7