TABLE 5.
Mobilization of pAM401 derivative plasmids by pHTβa
| Plasmid | Transfer frequency (no. of conjugants/donor)
|
|||
|---|---|---|---|---|
| Broth
|
Filter
|
|||
| Vmr | Cmr | Vmr | Cmr | |
| pAM401 | 1.2 × 10−4 | <10−7 | 4.7 × 10−2 | <10−8 |
| pAM401::IR5-1 | 6.0 × 10−5 | 4.1 × 10−5 | 5.3 × 10−2 | 1.1 × 10−1 |
| pAM401::IR5-2 | 8.0 × 10−4 | 3.8 × 10−5 | 3.2 × 10−2 | 1.0 × 10−1 |
| pAM401::IR5-3 | 1.1 × 10−4 | <10−7 | 2.5 × 10−2 | 3.2 × 10−4 |
| pAM401::IR5-4 | 1.1 × 10−4 | 5.1 × 10−5 | 4.0 × 10−2 | 1.3 × 10−1 |
| pAM401::IR5-5 | 1.4 × 10−4 | 6.9 × 10−5 | 2.5 × 10−2 | 1.2 × 10−1 |
| pAM401::IR5-6 | 9.0 × 10−5 | 3.0 × 10−5 | 4.4 × 10−2 | 1.1 × 10−1 |
| pAM401::IR5-7 | 1.6 × 10−4 | 6.7 × 10−5 | 3.1 × 10−2 | 1.2 × 10−1 |
| pAM401::IR5-8 | 7.0 × 10−5 | <10−7 | 4.8 × 10−2 | <10−8 |
| pAM401::IR5-9 | 1.4 × 10−4 | 4.2 × 10−5 | 3.0 × 10−2 | 1.2 × 10−1 |
| pAM401::IR5-10 | 1.8 × 10−4 | <10−7 | 2.7 × 10−2 | 2.5 × 10−4 |
a
The mating experiment was performed using E. faecalis UV202 as a donor carrying two plasmids and E. faecalis JH2SS as a recipient strain. The donor strains harbored both pHTβ (Vmr) as a mobilizer plasmid and each of the pAM401 derivatives (Cmr) containing various segments of the pHT plasmid as a tester plasmid. Each mating experiment was carried out in triplicate.