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Binding of B. subtilis LexA to potential SOS promoters. Mobility shift assays were conducted with purified LexA, radiolabeled recA promoter DNA (5 to 10 nM), and a 5- to 50-fold molar excess of the indicated promoter DNA as described in Materials and Methods. The lower and upper bands correspond to unbound and LexA-bound recA promoter DNA, respectively. Lanes with no LexA protein or competitor DNA added are indicated.
