TABLE 3.
Gene | Operator sequencea | Positionb | No. of mismatches | Kd (nM)c |
---|---|---|---|---|
LexA binding sites | ||||
fliP | aaAACgTAaGTTCG | Inside (+54) | 4 | >200 |
rpe | CGAACATgTtTTCG | Inside (+369) | 2 | >200 |
sigH | CGAAacTtTGTTCG | Inside (+127) | 3 | >200 |
ylbC | aGAACATAgGTTCc | Inside (+46) | 3 | 9.2 |
yonT | CGAACATAaGTTtt | −320 | 3 | 16 |
yqkA | gaAACtTgTGTTCG | Inside (+535) | 4 | >200 |
Sites that do not bind LexA | ||||
cysS | gaAACATtcGTTCc | Inside | 5 | |
leuB | tGAAaATATGTTCG | Inside | 2 | |
nprE | aGAACATATtTTCc | Inside | 3 | |
resE | gGAAttTATGTTtG | Inside | 4 | |
rpoC | CGAACgcATtTTCG | Inside | 3 | |
rsbU | gGAACtTtaGTTCc | Inside | 5 | |
xylR | gGAACAatcGTTCt | Inside | 5 | |
yaaC | gaAACATtTaTTCG | Inside | 4 | |
ybaL | aGAAtATgTGTTtG | Inside | 4 | |
yobR | CaAACAcATaTTCG | Inside | 3 | |
yqbA | CGAAagTATGTTCa | Inside | 3 | |
yqeV | aGAAttTATGTTCt | Inside | 4 | |
yurX | CGAACATAaGTTaa | Inside | 3 |
Nonconsensus nucleotides are shown in lowercase type, consensus nucleotides are shown in uppercase, and destabilizing mismatches are shown in boldface type.
Location of the 3′ end relative to the ATG codon of the respective gene.
Apparent binding constant.