FIG. 2.

capA, capB, capC, and capE are each necessary for PGA synthesis. (A and C) PGA was detected by dot blot. Bacteria were loaded directly on a nitrocellulose membrane and the membranes were probed with specific anti-PGA antibodies. (A) Strains RPG1, RTC50 (capE), and RTC50 complemented with capE. (B) Testing for CapD in RPG1, RTC10 (capB), RTC20 (capC), RTC30 (capA) and RTC40 (capD) strains. The same quantity of each sample was loaded. The Western blot membrane was probed with anti-CapD antibodies. (C) Strains RPG1, RTC10, RTC20, RTC30, and RTC40.