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. 2005 Nov;187(22):7765–7772. doi: 10.1128/JB.187.22.7765-7772.2005

FIG. 3.

FIG. 3.

CapE, the product of the 5th gene of the cap operon, is a membranous peptide. (A) Strains RPG1 and RTC50 were tested for CapB, CapA, and CapD. The same quantities of protein were loaded for each sample. CapB, CapA, and CapD were revealed by the corresponding specific antibodies. (B) RT-PCR was carried out on RNA from strain RTC50 using capEs/capEa (1), capEs/capEinternea (2), capD-RT2s/capDa (3), and capD-RT2s/capEinternea (4) primers. (C) Localization of the CapE peptide: cytoplasm (Cyto), membranous (Mb), and peptidoglycan (PG)-enriched fractions were separated. CapE was revealed by Western blot with specific anti-CapE antibodies.