TABLE 2.
pgsA and pgsE are necessary to complement a capE mutant straina
| Complementing gene | RTC50 (ΔcapE)
|
RTC30 (ΔcapA)
|
||
|---|---|---|---|---|
| Light microscopy | Petri dishes | Light microscopy | Petri dishes | |
| capE | + | + | ND | ND |
| ywtC | − | − | ND | ND |
| ywtB or pgsA | − | − | + | + |
| ywtB-ywtC or pgsA-ywtC | + | − | + | + |
a
Colonies on petri dishes were smooth (+) or rough (−), indicative of capsulation. The presence of PGA surrounding the bacteria was also determined by light microscopy in the presence of India ink. Capsulated bacteria present a white halo (+); others do not (−). The ywtC and pgsA genes are from B. subtilis, and the ywtC and ywtB genes are from B. licheniformis. ND, not done.