TABLE 1.
Strains useda
| Strain | Genotype | Plasmid |
|---|---|---|
| NH2504 | cobT714::MudJr2 cobP712::Tn10dGn | pJBREScI |
| NH2585 | zeh-754::Tn10 | pUC19 |
| NH2589 | zib-6794::Tn10 gyrB652 | pUC19 |
| NH2598 | cobT714::MudJr2 cobP712::Tn10dGn zib-6794::Tn10 gyrB652 | pJBREScI |
| NH2689 | hsdS gal attB::DE3 | pRC04 |
| NH2691 | hsdS gal attB::DE3 | pRC06 |
| NH3432 | SMT1556::MudJr2 (Mud547) zij-8267::Tn10dGn | pJBREScI |
| NH3435 | SMT1556::MudJr2 (Mud547) zij-8267::Tn10dGn zib-6794::Tn10 gyrB652 | pJBREScI |
| NH2936 | zbj-8906::MudJr2 zbj-8907::Tn10dGn | pJBREScI |
| NH3440 | zbj-8906::MudJr2 zbj-8907::Tn10dGn zib-6794::Tn10 gyrB652 | pJBREScI |
| NH3500 | (STM1553 <FRT> STM1554) | pCP20 |
| NH3501 | (smpB <FRT> STM2689) | pCP20 |
| NH3502 | (STM3261 <FRT> STM3262) | pCP20 |
| NH3503 | (atpI <FRT> gidB) | pCP20 |
| NH3504 | (STM1553 <FRT res lacZYA gen res FRT> STM1554) | pJBREScI |
| NH3505 | (smpB <FRT res lacZYA gen res FRT> STM2689) | pJBREScI |
| NH3506 | (STM3261 <FRT res lacZYA gen res FRT> STM3262) | pJBREScI |
| NH3507 | (atpI <FRT res lacZYA gen res FRT> gidB) | pJBREScI |
| NH3508 | zib-6794::Tn10 gyrB652 (STM1553 <FRT res lacZYA gen res FRT> STM1554) | pJBREScI |
| NH3509 | zib-6794::Tn10 gyrB652 (smpB <FRT res lacZYA gen res FRT> STM2689) | pJBREScI |
| NH3510 | zib-6794::Tn10 gyrB652 (STM3261 <FRT res lacZYA gen res FRT> STM3262) | pJBREScI |
| NH3511 | zib-6794::Tn10 gyrB652 (atpI <FRT res lacZYA gen res FRT> gidB) | pJBREScI |
a
All strains are derivatives of S. enterica serovar Typhimurium strain LT2 except for NH2689 and NH2691, which are E. coli B derivatives. All strains were constructed for this study.