TABLE 2.

Effect of gyrB652 mutation on resolution in different chromosome regions^a

Strain	Region	gyrB	Deletions in exponential growth (%)	Deletions in stationary growth (%)
NH2504	CS 43	WT	90	97
NH2598	CS 43	gyrB652	10	49
NH3432	CS 33	WT	82	99
NH3435	CS 33	gyrB652	3	10
NH2936	CS 19	WT	90	99
NH3440	CS 19	gyrB652	18	65
NH3504	CS 33	WT	75 ± 5	97 ± 3
NH3508	CS 33	gyrB652	3.9 ± 0.2	23 ± 1
NH3505	CS 58	WT	92 ± 2	95 ± 1
NH3509	CS 58	gyrB652	7 ± 2	80 ± 5
NH3506	CS 71	WT	91 ± 2	98 ± 1
NH3510	CS 71	gyrB652	13 ± 1	46 ± 4
NH3507	CS 85	WT	93 ± 4	94 ± 6
NH3511	CS 85	gyrB652	22 ± 3	93 ± 3

^aResolution assays were carried out as described previously (37, 64). Briefly, cells grown at 30°C to mid-log phase were shifted to 42°C for 10 min to induce expression of Tn3 resolvase from pJBREScI. Cells were diluted into LB, incubated at 30°C in LB for an hour to allow chromosomal segregation, and plated on selective medium containing X-Gal. Recombinant colonies appearing on plates incubated at 30°C were scored after 3 to 5 days. The recombination numbers reported here represent average values from three or more independent cultures. Variation between replicas was less than 15% of the reported values.