TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Streptococcus dysgalactiae subsp. equisimilis | Human serogroup C | |
| H46A | Cov− (K102 amber) Fas+ | 3; this study |
| H46A(pMcovRSF) | Cov+ Fas+; Emr | This study |
| H46AdexB::pVAcovRSNZdI | Cov+ Fas+; Emr | This study |
| H46AdexB::pVAcovRSNZdII | Cov+ Fas+; Emr | This study |
| H46AfasA::pFW15fasA1 | Cov− Fas−; Emr | This study |
| H46AfasA::pFW14fasA2 | Cov− Fas−; Cmr | This study |
| H46AfasA::pFW15fasA1(pFasAXSF) | Cov− Fas+; Emr Kmr | This study |
| H46AdexB::pVAcovRSNZdII, fasA::pFW5fasA1 | Cov+ Fas−; Emr Spcr | This study |
| Streptococcus pyogenes | Human serogroup A | |
| NZ131 | Cov+ Fas+ | J. J. Ferretti |
| NZ131covR::pFC1 | Cov− Fas+; Spcr | 27 |
| NZ131covR::pFC1, covR::pVAcovRSNZ | Cov+ Fas+; Spcr Emr | This study |
| NZ131covR::pFC1, fasA::pFW15fasA1 | Cov− Fas−; Spcr Emr | This study |
| Plasmids | ||
| pMG36, pMG36e | Vectors; Kmr and Emr respectively | 31 |
| pMcovRSF | pMG36e containing covRSF370 under its natural promoter; Emr | This study |
| pVA8912 | Suicide vector; Emr | 26 |
| pVAcovRSNZ | pVA8912 containing covRSNZ131 under its natural promoter; Emr | This study |
| pVAcovRSNZdI | pVA8912 containing covRSNZ131 under its natural promoter and the 3′ end of dexBH46A (orientation I) for genomic integration into H46A; Emr | This study |
| pVAcovRSNZdII | As pVA covRSNZdI but 3′ end of dexBH46A in orientation II; Emr | This study |
| pFW5, pFW14, pFW15 | Vectors; Spcr, Cmr, Emr, respectively | 24 |
| pFW5fasA1 | pFW5 containing an internal fragment of fasAH46A; Spcr | This study |
| pFW15fasA1 | As pFW5 fasA1; Emr | This study |
| pFW14fasA2 | pFW14 containing an internal fragment of fasAH46A; Cmr | This study |
| pFasAXSF | pMG36 containing fasAXSF370 under vector promoter P32; Kmr | This study |