TABLE 2.
Subclasses of anti-LPS antibody after immunization with LPS-GBOMP vaccinea
| Antibody | Sera no. (wk after second dose) | ELISA antibody units (mean ± SD)b
|
|||
|---|---|---|---|---|---|
| IgG1 | IgG2a | IgG2b | IgG3 | ||
| Anti-B. melitensis LPSc | 31-40 (2) | 22,341 ± 10,983 | 217 ± 113 | 385 ± 322 | 2,111 ± 829 |
| 41-50 (4) | 23,805 ± 9,529 | 461 ± 217 | 288 ± 92 | 1,413 ± 393 | |
| Anti-Y. enterocolitica LPSd | 31-39 (2) | 19,241 ± 13,837 | 2,579 ± 4,743 | 3,900 ± 7,409 | 3,934 ± 2,348 |
| 41-49 (4) | 24,396 ± 12,904 | 770 ± 714 | 1,022 ± 1,377 | 4,456 ± 2,235 | |
a
Mice were immunized twice intranasally 4 weeks apart with B. melitensis LPS-GBOMP vaccine. Sera were collected 2 or 4 weeks after the second dose for ELISA.
b
Data are expressed in OD units. Negative control sera run simultaneously in these assays had titers of less than 1 OD unit.
c
The B. melitensis LPS contained 5.5% protein.
d
The Y. enterocolitica O:9 LPS contained 0.1% protein. At the time of running Y. enterocolitica O:9 LPS ELISA, we ran out of sera 40 and 50; therefore, there were 9 instead of 10 sera for each set.