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. 2025 Dec 15;16:1847. doi: 10.1038/s41598-025-31605-x

Table 2.

Linearity, calibration data, and system suitability for SFS and LDS determination using the proposed HPLC method (n = 6).

Parameters SFS LDS
Linearity range (µg.mL-1) 10.00–60.00 2.25–13.50
Regression equation Y = 30.8X + 86.9 Y = 66.6X + 62.8
Coefficient of determination (R2) 0.999 0.999
LOD (µg.mL-1) 0.72 0.23
LOQ (µg.mL-1) 2.18 0.72
tR ± SD (min.) 1.85 ± 0.1 2.97 ± 0.3
N 7600 2760
α 3.5
Rs 4.0