TABLE 3.
IrgA and VctA promote utilization of enterobactin
| Indicator strain | Relevant phenotype | Zone of stimulation (mm) witha:
|
|||
|---|---|---|---|---|---|
| DH5α (enterobactin) | Lou15 (vibriobactin) | Heme | Ferrichrome | ||
| CA40130N | Parent | 17 | 26 | 18 | 24 |
| ARM316 | IrgA− | 14 | 24 | 17 | 22 |
| AGO310 | VctA− | 16 | 26 | 18 | 23 |
| ARM616/pWKS30 | VctA− IrgA− | 0 | 25 | 17 | 28 |
| ARM616/pCAT119 | VctA+ IrgA− | 16 | 28 | 16 | 26 |
| ARM616/pCAT121 | VctA− IrgA+ | 16 | 19 | 16 | 25 |
a
Cultures of the indicator strains were seeded into L agar containing 100 μg of EDDA per ml. E. coli strain DH5α (as a source of enterobactin), V. cholerae strain Lou15 (as a source of vibriobactin), heme, or ferrichrome was spotted onto the medium. The zone of growth was measured at 18 h after inoculation.