TABLE 1.

List of peptides that allowed generation of long-term CTL lines

Protein	Peptide name	Sequence and position	Binding motif	Prediction scorea	Expt binding to MHC class Ib
MOMP	7087	67 GDYVFDRI 74	H-2Kb	22	+++
MOMP	7093	319 SLLGNATAL 327	H-2Db	27	+
Pomp5	9165	283 QAVANGGAI 291	H-2Db	25	+++
Omp2	8482	59 RGAFCDKEF 67	H-2Dd	18	ND
Omp2	8511	85 CYGRLYSVKV 94	H-2Kd	18	ND
Hsp60	7233	7 KYNEEARKKI 16	H-2Kd	24	ND
Hsp60	7239	32 GPKGRHVVI 40	H-2Ld	18	ND
Ovalbuminc	7548	257 SIINFEKL 264	H-2Kb	25	+++
β-Galactosidasec	9339	876 TPHPARIGL 884	H-2Ld	25	ND

^aThe binding capacity of the peptides was scored according to the presence of anchor amino acids and other amino acids frequently occurring in natural ligands (20).

^bPeptides possessing H-2^b-binding anchor residues were tested for the ability to bind to class I molecules in vitro as described in Materials and Methods. ND, not determined; +++, strong stabilization of MHC assembly by peptide; +, stabilization of MHC assembly by peptide.

^cPreviously described CTL epitopes of ovalbumin (22) and E. coli β-galactosidase (4) were used as controls.