TABLE 2.
Induction of proliferation and secretion of TNF-α and IFN-γ by peptide-specific CTL lines in response to stimulation with C. pneumoniae-derived peptides
| CTL line specific for peptide | PIa | TNF-α concn (pg/ml)b | IFN-γ concn (pg/ml) |
|---|---|---|---|
| 7087 (MOMP) | 3 | 230 | 11,000 |
| 7093 (MOMP) | 69 | 8,700 | 33,000 |
| 8482 (Omp2) | 52 | 1,400 | 8,000 |
| 8511 (Omp2) | 7 | 11,000 | 118,000 |
| 9165 (Pomp5) | 88 | 2,400 | 23,000 |
| 7233 (Hsp60) | 13 | 4,600 | 23,000 |
| 7239 (Hsp60) | 3 | 240 | 8,000 |
| 7548 (Ovalbumin) | 92 | 4,100 | 13,000 |
| 9339 (β-galactosidase) | 98 | 6,200 | 4,000 |
a
Three-day proliferation assays based on the incorporation of [3H]thymidine during the last 16 to 18 h were performed at an E/T cell ratio of 1 and a peptide concentration of 1 μg/ml. The PI was calculated as follows: PI = (peptide-induced proliferation − background proliferation)/background proliferation.
b
Secretion of cytokines was determined by ELISA in samples of cell culture supernatants taken from the proliferation assay wells 48 h after peptide stimulation.