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. 2002 Jul;70(7):3427–3432. doi: 10.1128/IAI.70.7.3427-3432.2002

FIG. 2.

FIG. 2.

Localization of an epitope recognized by 2B3. (A) Trypsin-treated (lanes 1, 3, and 5) or nontreated (lanes 2, 4, and 6) DNT was sujected to Western blot analysis with 2B3 (lanes 1 and 2) or 1A3 (lanes 3 and 4). Total proteins electrotransferred on the membrane were stained with Coomassie brilliant blue R-250 (lanes 5 and 6).(B) The lysates of E. coli expressing DNT mutants were subjected to SDS-PAGE (upper panel) and Western blot analysis with 2B3 (lower panel). (C) A schematic representation of wild-type (wt) and deletion mutants of DNT. The results of the Western blot analyses are summarized on the right. The numbers in parentheses indicate the positions of amino acids covering the peptides or deletions (Δ). The C-terminal ends of the tryptic 60- and 90-kDa fragments were not identified. (D) His-DNTwt (lanes 1 and 5), His-DNT R44G (lanes 2 and 6), His-DNT R44S (lanes 3 and 7), and His-DNT R44K (lanes 4 and 8) were subjected to SDS-PAGE and immunoblotted (IB) with 1A3 (lanes 1 to 4) or 2B3 (lanes 5 to 8).