FIG. 3.

The DNT-induced formation of actin stress fibers in MC3T3-E1 cells microinjected with antibodies. The cells were microinjected with buffer alone (A to D), anti-DNT polyclonal antibody (E and F), or 2B3 (G and H). After incubation with (C to H) or without (A and B) 5 ng of DNT/ml for 20 h, the cells were stained for actin cytoskeletons (B, D, F, and H). Microinjected cells were distinguished by staining with Alexa 488-antibody against rabbit IgG microinjected along with the test samples (A, C, E, and G).