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Effects of hemozoin on DNA vaccine-induced antibody isotypes. Antibody titers to rPfs25 were measured by ELISA. Four BALB/c mice for each group were analyzed individually, and the IgG2a/IgG1 ratio was calculated for each mouse. Averages + standard deviations are shown for each group. Results are representative of two different animal experiments at different times. Statistical significance (∗) was determined by Student's t test (P < 0.03).
