FIG. 2.

Transcription of incA is comparable in 32 and 37°C cultures. HeLa cell monolayers were infected at an MOI of 5 with C. trachomatis L2 and cultivated at either 32 or 37°C for 24 h. Whole-culture RNA was harvested, and incA and incG messages were amplified by RT-PCR. 16S rRNA was amplified as a control for RNA quality and to normalize analyzed samples. Products were amplified from C. trachomatis L2 genomic DNA and from RNA harvested from uninfected HeLa cells as positive and negative controls, respectively. Reactions were performed in the presence (+) or in the absence (−) of RT, and products were resolved in 1.5% agarose gels and stained with ethidium bromide.