FIG. 2.

Dot blot hybridization between individual M. avium cosmid clones (the same clones as in Fig. 1) and a cDNA mixture prepared from M. avium grown for 110 h in human macrophages (cDNA mixture 8 [Table 1]). The M. avium cDNA mixture was prepared by three rounds of SCOTS and four rounds of enrichment for cDNA molecules corresponding to M. avium genes expressed during growth in macrophages. Labeling of the cDNA probe and hybridization and wash conditions were as described in Materials and Methods.