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IFN-γ production by in vitro-stimulated spleen cells from mice immunized with Ag2/PRA(1-194), Ag2/PRA(19-194), or Ag2/PRA(1-18), each ligated to pVR1012. Spleen cells were pooled from groups of 10 mice and stimulated with the full-length GST-Ag2/PRA recombinant protein (A) or the synthetic signal peptide (B) for 48 h, and the supernatants were assayed for IFN-γ by an ELISA.
